Cloned (Comment) | Organism |
---|---|
cloned into the pMyr vector and expressed as fusion protein with a myristylation sequence, which anchors the target protein to the yeast membrane. Fusion proteins (pSOS/SalAT and pMyr/SalR) coexpressed in the Saccharomyces cerevisiae cdc25H strain. SalR amplified from the recombinant plasmid SalR/pQE-30. The 0.9-kb DNA fragment ligated into an NheI/EcoRI-digested pET28a expression vector. SalR/pET28a expression construct transformed into Escherichia coli BL21(DE3)RIL | Papaver somniferum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | direct influence of a reduction in salAT transcript level by RNAi on the transcription of salR is not evident | Papaver somniferum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Papaver somniferum | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tagged SalR purified with a cobalt affinity resin | Papaver somniferum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Papaver somniferum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
salutaridine + NADPH + H+ | interaction between SalR and SalAT in morphine biosynthesis | Papaver somniferum | (7S)-salutaridinol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SalR | - |
Papaver somniferum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Papaver somniferum |